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Originally Posted by ecriture d'adulte
Nobody doubts that they are capable of engineering A virus and lying about it after the fact if the lie benefits them. It's that this particular virus has all the hallmarks of naturally evolving in nature and none of the signatures of lab engineering.
This is false. Nothing so far precludes the escape from a lab. When combined with the false and misleading origin stories given by the Chinese in the early days of the pandemic, and the odd goings-on at the lab itself, there is a strong consilience argument that the virus indeed escaped from a lab.
-receptor binding motif in Sars-Cov-2 that creates the furin cleavage site for human transmission is highly similar to experiments that we know have been done in the Wuhan laboratory
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Shi ZhengliÂ’s group was creating chimeric constructs as far back as 2007 and as recently as 2017, when they created a whole of 8 new chimeric coronaviruses with various RBMs. In 2019 such work was in full swing, as WIV was part of a $3.7 million NIH grant titled Understanding the Risk of Bat Coronavirus Emergence. Under its auspices, Shi Zhengli co-authored a 2019 paper that called for continued research into synthetic viruses and testing them in vitro and in vivo
-Cov-2 is 96% identical to a Yunnan bat strain, bats that are not native to Wuhan, that were not found at the seafood market, bats that are native to Yunnan province, 1800km away from Wuhan, bats that have been collected for coronavirus analysis by the Wuhan lab as far back as 2013
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When the CoV2 genome was just sequenced and made publicly available on January 10, 2020, it was a riddle, as no closely related strains were known. But quite quickly, on January 23, Shi Zhengli released a paper indicating that CoV2 is 96% identical to RaTG13, a strain which her laboratory had previously isolated from Yunnan bats in 2013. However, outside of her lab, no one knew about that strain until January 2020.
-the other major part of the identity of the cov-2 virus is pangolin in nature, and can be traced to sick pangolin confiscation and sampling done in 2017
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We received frozen tissue (lungs, intestine, blood) samples that were collected from 18 Malayan pangolins (Manis javanica) during August 2017-January 2018. These pangolins were obtained during the anti-smuggling operations by Guangxi Customs. Strikingly, high-throughput sequencing of their RNA revealed the presence of coronaviruses in six (two lung, two intestine, one lung-intestine mix, one blood) of 43 samples. With the sequence read data, and by filling gaps with amplicon sequencing, we were able to obtain six full or nearly full genome sequences — denoted GX/P1E, GX/P2V, GX/P3B, GX/P4L, GX/P5E and GX/P5L — that fall into the 2019-CoV2 lineage (within the genus Betacoronavirus) in a phylogenetic analysis (Figure 1a).
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More notable, however, was the observation of putative recombination signals between the pangolins coronaviruses, bat coronaviruses RaTG13, and human 2019-CoV2 (Figure 1c, d). In particular, 2019-CoV2 exhibits very high sequence similarity to the Guangdong pangolin coronaviruses in the receptor-binding domain (RBD; 97.4% amino acid similarity; indicated by red arrow in Figure 1c and Figure 2a), even though it is most closely related to bat coronavirus RaTG13 in the remainder of the viral genome. Bat CoV RaTG and the human 2019-CoV2 have only 89.2% amino acid similarity in RBD. Indeed, the Guangdong pangolin coronaviruses and 2019-CoV2 possess identical amino acids at the five critical residues of the RBD, whereas RaTG13 only shares one amino acid with 2019-CoV2 (residue 442, human SARS-CoV numbering)
-furin cleavage site has no close relatives, suggesting it might have been bioengineered
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The closest relative with a furin site is the HKU5 strain, isolated by the Shi Zhengli team in 2014 in Guangzhou from bats of the genus Pipistrellus (added to GenBank in 2018). But it is a very distant relative — their spike proteins share only 36%.
So the virologists are puzzled. Where did this 12 nucleotide insert come from? Could it be lab-made? Well, virologists have studied furin sites in coronaviruses for decades, and have introduced many artificial ones in a lab. For example, an American team had inserted RRSRR into the spike protein of the first SARS-CoV back in 2006
-ratg13 is 100% identical to rabtcov/4991, found in Yunnan bats in 2013
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this preprint that alleges that RaTG13 is, in fact, RaBtCoV/4991 (KP876546), which Shi Zhengli had previously reported discovering in an abandoned mineshaft in Yunnan in 2013. There indeed are several reasons to think so. First and foremost, the only published sequence for RaBtCoV/4991 is 100% identical to that of RaTG13 at the nucleotide level, albeit being just a 370-bp stretch of the RdRp gene:
-several Wuhan lab workers became ill with pneumonia in autumn 2019
-CCP has refused any interviews with these lab workers
-CCP had delayed, to worldwide criticism, any WHO investigation
-CCP jumping all over theories the origin was in a different country