yes it is normal that your red line is in minus. This is nothing to worry about. In fact, it will likely move into positive once you reduce your losses in the blinds.
to check the profitability of your isolation bets: click on filters. In main filters, under preflop action facing player, deselect everything except one limper. then click on the more filters tab, click on pfr = true. add this filter, save and close. go to the reports tab and go to the position page.
This shows you the overall profitability of our iso strat v one limper by position. If you have a big sample (several hundred hands per position), you can assume that your basic strat is profitable if you are green in all positions. the next step is to click on filters, go to more filters, scroll down to flop filters, and select saw flop = true. add this filter, save and close. back on your position page, you are now analyzing your post flop play in pots where you isolated and got called.
Once you are done with that, go back to main filters, deselect one limper, and select 2+ limpers. Go back to more filters and delete saw flop = true. save and close. analyze your overall strategy by position. Then go back into more filters and add back in saw flop = true to check your postflop play.
When you are looking at one limper and pfr = true, you should see a wr of at least 300bb/100 hands. You are probably making mistakes if you have a big sample of hands and you are below this number.
when you add saw flop = true, your wr should go up to about 400bb/100. If not, you probably are making mistakes.
I looked over the stats you posted. with two exceptions, they are very solid. The leaks I see from your stats are:
you are losing way too much money in the blinds. there are a few usual causes of this. The one that is most likely for you is that you are not winning enough of the BB hands where you get a free look at the flop. We like to try to win more than our share of these pots, and I doubt that you are. My guess is that if you add W$WSF to your position page, then filter vpip = false, saw flop = true, that you will see a W$WSF around 24% If so, that is a significant leak, and you should be aiming to increase this number to around 31%.
another possible leak you have in the blinds is cold calling out of the blinds. again from the position page, go to more filters and add filter did cold call = true. Ideally, this number should be positive. Realistically, though yours is likely to be between -50bb/100 and break even. The further you are from a positive number here, the bigger the leak. You should also run this filter for the SB.
The last thing I saw is that your overall W$WSF is on the low side, indicating that you may be a little weak tight. If you cross reference this data with the fact that in 3 or 4 positions you have a W$SD below 50%, it indicates that you are calling to showdown too much, rather than betting or folding, and the net result is that you are losing too many pots. Most of these are probably smallish pots, as you were below 50% in both the blinds. So under more filters, you should filter for "river call = true and river check/call = true" and carefully study the marginal hands you lost at showdown with, with an eye toward betting or folding on earlier streets.
Lastly, you were under 50% W$SD on the button. This is a big leak, and, again, it indicates that you are taking far too many low quality hands to showdown. these are probably your steals where the villain value bets the river. To confirm this, go to main filters, deselect everything other than "unopened" then go to more filters, add pfr = true and saw flop = true--these are your steals where the villain defended. You will probably have a negative win rate here, indicating that you are calling down too lightly with your steals. review these hands carefully looking for hands you should have folded instead of calling. If this number is positive, meaning that you are playing your steals profitably, then the next most likely thing is that you are losing money cold calling IP. If it is neither of these things, pm me and I will take a look for you.