I am in search of software that is able to accurately calculate card removal in 6 max NLHE if we input proper opening hand ranges.

Here is an example of a hand that I would be interested in calculating:

100BB 6 max Game NLHE

UTG folds, HJ folds, CO folds, BTN folds. SB has 99 and raises 3x, BB has AKo and 3bets, 99 4bets and AKo shoves AI. 99 calls.

Usually the odds in this hand would be AKo 44% and 99 around 56% but consider removal effects. It is more likely that one of 8 cards that was folded was a 9, and not an Ace or a King. This means that AKo actually has more equity then normal because although the 8 cards folded are not known, they can be put into probability buckets based on starting ranges.

Can anyone point me in the right direction regarding software that quantifies this? Or has anyone done any extensive study on the effects card removal has on equities.

what you're looking for is Card Runners EV from scylla he has a thread on here. I use it all the time. He also makes flopzilla but it has a more limited form of card removal (you can specify what 2 cards you want to remove)