my home game last night (1/1 because we are short on chips), all three of us are about 400BB deep.

villains are passive fishes who can bluff now and then. this is my first game with them. my image is of a competent player.
they are both not that comfortable with having so much money in front of them.

1/1 NL (7 handed)
Hero (UTG) (400)
UTG+1 (400)
SB (400)

Hero is dealt QQ

Hero raises to 5, UTG+1 raises to 10, folds, SB calls 10, Hero raises to 25, UTG+1 calls, SB calls.

(5 is a standard raise with no limpers in front of you. obviously i didn't like his min raise as this usually signifies a very strong hand. if i was left heads up with UTG+1 i might of just called and kept the pot small but i didn't want another player with us so i decided to 4bet also in order to isolate. i put UTG+1 on TT+/AQ+ (very strong hands in his eyes), my thought was he would probably call with all of those holding and if he has KK/AA he would probably 5bet and let me know that i'm beat. i should of raised more.)

Flop (75) 9,5,2 rainbow
SB checks, Hero bets 50, UTG+1 calls, SB folds

(almost confident i have the best hand - i'm betting for value, should of bet the pot as i believe even if he has AK he's gonna peal. when he calls i'm putting him on AK/JJ.)

Turn (175) A

Hero bets 50, UTG+1 calls

(i'm scared but don't want to show weakness. i know villain doesn't understand betting sizes and he sometimes bets the same each street. when he calls i think i might be beat)

River (275) K

Hero ??

(c/f, b/f, c/c ?? - checking is looked upon as very weak and might induce a bluff).

would appreciate comments on all streets

I like four betting larger preflop, more in the lines of $40 or $50. I like your flop and turn play, now I would casually check the river and pray that he does the same.

Some may disagree, but I don't see anything wrong with just calling his minraise since you're out of position. If you are going to raise, I'd make it more like 35 or 40; 25 is just begging to get called.

The flop seems fine. On the turn, I'd probably check. I realize this opens you up to getting bluffed off what could be the best hand; however you said villian was passive and not comfortable playing this deep. Given that I doubt he's going to turn JJ into a bluff at this point. If you check and he bets you can safely fold.

On the river I can't see doing anything but check/folding. Given your description I'm sure he'll gladly check behind if he has worse than you do.

Looks like everything has already been said.

If you want to 4bet here, making it 50 seems like a good amount. Flop, good. Turn, good. River, check/fold. Sucks they got there

Are we checking the river because it was a K or because opp called the turn?

If checking in this game induces a bluff, why not bet another 50 on the river for value + protection in a 275 pot.

We're only getting called by hands that beat us.

OP said villian bluffs now and then but also that he's passive and uncomfortable playing deep. From this description, I take it to mean that he'll occasionally bet a busted flush draw in a $30 pot, not bluff in a huge 4bet pot 400bb deep. I really think we're beat and putting any more money in the pot is a mistake.