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Now it's at 4.458 per hand after 40k seconds and keeps fluctuating with every new iteration. Is this normal for preflop sims?
The preflop solver operates on lower accuracy (to save memory) than the postflop one that's why this may happen in some spots. It's already improved a bit in dev but not completely solved. Here are things to keep in mind:
1)It's a very bad idea to use multiple bet sizes postflop when solving preflop trees. Not only it doesn't influence preflop ranges almost at all but it makes things very difficult for the preflop solver. It's especially important to avoid overbets (so "add all-in" checkbox)
2)It's easier for the solver if actions which go to 0% frequency during solving are not included in the tree.
3)Sometimes you just can't reach very good accuracy (bb/100 wise), this happens mainly in forced 3bet/4bet scenarios where the starting pot is very big.
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I set the accuracy to 0.35% as I read earlier from this thread that it's generally good enough for postflop spots. Does this also apply to preflop sims?
It's not easy to tell what the starting pot is in the preflop scenarios (it's unclear how to treat blinds) it makes more sense to operate on bb/100 terms there. You should aim at 2bb-3bb/100 accuracy. You can usually go lower than that with enough time but sadly not always.